Oestradiol measured by liquid chromatography-mass spectrometry (LCMS) is the gold standard method for oestradiol quantification, offering far greater accuracy and specificity than conventional immunoassay methods — particularly at very low concentrations.
Standard immunoassay oestradiol tests can produce unreliable results in postmenopausal women, men, children, and individuals on hormone therapy — precisely the groups where accurate measurement matters most. LCMS eliminates cross-reactivity with structurally similar hormones and avoids interference from high-dose biotin supplementation. It is the preferred method for anyone requiring precise hormonal assessment, including those on testosterone therapy, hormone replacement therapy, or undergoing IVF monitoring.
FAQs
Why choose LCMS oestradiol over standard oestradiol?
LCMS eliminates interference from biotin supplementation, cross-reacting steroids, and heterophilic antibodies. It is significantly more accurate at low concentrations, making it essential for postmenopausal women, men, children, and anyone on hormone therapy where precision matters.
Does LCMS require a different blood sample?
The collection process is the same as any blood test. The difference is in how the laboratory processes and measures the sample. Specify LCMS when ordering to ensure the correct method is used.
Should I stop biotin supplements before testing?
Yes. High-dose biotin supplementation interferes with many immunoassay hormone tests. LCMS oestradiol is not affected by biotin, but it is still good practice to stop high-dose biotin for 2-3 days before any hormonal testing.
Is LCMS oestradiol available routinely?
LCMS oestradiol is available through specialist endocrinology laboratories and some private pathology services. It may be specifically requested when routine immunoassay results are discordant with clinical findings.
